EM specimen
| Vitrification | false |
| Staining | false |
| Instrument | FEI VITROBOT MARK IV |
6SGB |
mt-SSU assemblosome of Trypanosoma brucei |
This is a large structure. |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.3 |
| Software | ,EPU,Gctf,RELION,PHENIX |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
