6SGB

mt-SSU assemblosome of Trypanosoma brucei

This is a large structure.

EM specimen

Vitrificationfalse
Stainingfalse
InstrumentFEI VITROBOT MARK IV

EM Data Collection

MicroscopeFEI TITAN KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
DetectorFEI FALCON III (4k x 4k)

3D Reconstruction

MethodSINGLE PARTICLE
Resolution (Å)3.3
Software,EPU,Gctf,RELION,PHENIX
SymmetryC1
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION