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- PDB-7jz2: Succinate: quinone oxidoreductase SQR from E.coli K12 -

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Entry
Database: PDB / ID: 7jz2
TitleSuccinate: quinone oxidoreductase SQR from E.coli K12
Components(Succinate dehydrogenase ...) x 4
KeywordsELECTRON TRANSPORT/OXIDOREDUCTASE / Complex / Succinate: quinone oxidoreductase / SdhA / sdhB / SdhC / SdhD / ELECTRON TRANSPORT / ELECTRON TRANSPORT-OXIDOREDUCTASE complex
Function / homology
Function and homology information


plasma membrane succinate dehydrogenase complex / succinate dehydrogenase complex / succinate dehydrogenase (ubiquinone) activity / succinate dehydrogenase / cytochrome complex assembly / succinate dehydrogenase activity / anaerobic respiration / ubiquinone binding / iron-sulfur cluster binding / 3 iron, 4 sulfur cluster binding ...plasma membrane succinate dehydrogenase complex / succinate dehydrogenase complex / succinate dehydrogenase (ubiquinone) activity / succinate dehydrogenase / cytochrome complex assembly / succinate dehydrogenase activity / anaerobic respiration / ubiquinone binding / iron-sulfur cluster binding / 3 iron, 4 sulfur cluster binding / aerobic respiration / respiratory electron transport chain / tricarboxylic acid cycle / 2 iron, 2 sulfur cluster binding / 4 iron, 4 sulfur cluster binding / flavin adenine dinucleotide binding / go:0055114: / electron transfer activity / oxidoreductase activity / heme binding / integral component of membrane / plasma membrane / metal ion binding
Succinate dehydrogenase/Fumarate reductase transmembrane subunit / Succinate dehydrogenase, cytochrome b subunit, conserved site / Alpha-helical ferredoxin / Succinate dehydrogenase, flavoprotein subunit / Beta-grasp domain superfamily / Succinate dehydrogenase/fumarate reductase, flavoprotein subunit / Succinate dehydrogenase, hydrophobic membrane anchor / Succinate dehydrogenase, cytochrome b556 subunit / Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal / 4Fe-4S ferredoxin-type, iron-sulphur binding domain ...Succinate dehydrogenase/Fumarate reductase transmembrane subunit / Succinate dehydrogenase, cytochrome b subunit, conserved site / Alpha-helical ferredoxin / Succinate dehydrogenase, flavoprotein subunit / Beta-grasp domain superfamily / Succinate dehydrogenase/fumarate reductase, flavoprotein subunit / Succinate dehydrogenase, hydrophobic membrane anchor / Succinate dehydrogenase, cytochrome b556 subunit / Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal / 4Fe-4S ferredoxin-type, iron-sulphur binding domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / Succinate dehydogenase/fumarate reductase N-terminal / Fumarate reductase/succinate dehydrogenase, FAD-binding site / Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain superfamily / Fumarate reductase/succinate dehydrogenase, transmembrane subunit / 2Fe-2S ferredoxin-like superfamily / FAD/NAD(P)-binding domain superfamily / Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain superfamily / FAD binding domain / Fumarate reductase flavoprotein C-term / 2Fe-2S iron-sulfur cluster binding domain / 4Fe-4S dicluster domain / Succinate dehydrogenase/fumarate reductase iron-sulphur protein / FAD-dependent oxidoreductase 2, FAD binding domain / 2Fe-2S ferredoxin-type iron-sulfur binding domain / Succinate dehydrogenase/fumarate reductase type B, transmembrane subunit
Succinate dehydrogenase flavoprotein subunit / Succinate dehydrogenase cytochrome b556 subunit / Succinate dehydrogenase iron-sulfur subunit / Succinate dehydrogenase hydrophobic membrane anchor subunit
Biological speciesEscherichia coli (E. coli)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.5 Å
AuthorsLyu, M. / Su, C.-C. / Morgan, C.E. / Bolla, J.R. / Robinson, C.V. / Yu, E.W.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) United States
CitationJournal: Nat Methods / Year: 2021
Title: A 'Build and Retrieve' methodology to simultaneously solve cryo-EM structures of membrane proteins.
Authors: Chih-Chia Su / Meinan Lyu / Christopher E Morgan / Jani Reddy Bolla / Carol V Robinson / Edward W Yu /
Abstract: Single-particle cryo-electron microscopy (cryo-EM) has become a powerful technique in the field of structural biology. However, the inability to reliably produce pure, homogeneous membrane protein ...Single-particle cryo-electron microscopy (cryo-EM) has become a powerful technique in the field of structural biology. However, the inability to reliably produce pure, homogeneous membrane protein samples hampers the progress of their structural determination. Here, we develop a bottom-up iterative method, Build and Retrieve (BaR), that enables the identification and determination of cryo-EM structures of a variety of inner and outer membrane proteins, including membrane protein complexes of different sizes and dimensions, from a heterogeneous, impure protein sample. We also use the BaR methodology to elucidate structural information from Escherichia coli K12 crude membrane and raw lysate. The findings demonstrate that it is possible to solve high-resolution structures of a number of relatively small (<100 kDa) and less abundant (<10%) unidentified membrane proteins within a single, heterogeneous sample. Importantly, these results highlight the potential of cryo-EM for systems structural proteomics.
Validation Report
SummaryFull reportAbout validation report
History
DepositionSep 1, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 20, 2021Provider: repository / Type: Initial release

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Assembly

Deposited unit
A: Succinate dehydrogenase flavoprotein subunit
B: Succinate dehydrogenase iron-sulfur subunit
C: Succinate dehydrogenase cytochrome b556 subunit
D: Succinate dehydrogenase hydrophobic membrane anchor subunit
E: Succinate dehydrogenase flavoprotein subunit
F: Succinate dehydrogenase iron-sulfur subunit
G: Succinate dehydrogenase cytochrome b556 subunit
H: Succinate dehydrogenase hydrophobic membrane anchor subunit
I: Succinate dehydrogenase flavoprotein subunit
J: Succinate dehydrogenase iron-sulfur subunit
K: Succinate dehydrogenase cytochrome b556 subunit
L: Succinate dehydrogenase hydrophobic membrane anchor subunit
hetero molecules


Theoretical massNumber of molelcules
Total (without water)365,41836
Polymers355,47412
Non-polymers9,94424
Water0
1


TypeNameSymmetry operationNumber
identity operation1_5551
Buried area56500 Å2
ΔGint-592 kcal/mol
Surface area102960 Å2

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Components

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Succinate dehydrogenase ... , 4 types, 12 molecules AEIBFJCGKDHL

#1: Protein Succinate dehydrogenase flavoprotein subunit


Mass: 64502.766 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / Strain: K12 / References: UniProt: P0AC41, succinate dehydrogenase
#2: Protein Succinate dehydrogenase iron-sulfur subunit


Mass: 26800.912 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / Strain: K12 / References: UniProt: P07014, succinate dehydrogenase
#3: Protein Succinate dehydrogenase cytochrome b556 subunit / Cytochrome b-556


Mass: 14313.100 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / Strain: K12 / References: UniProt: P69054
#4: Protein Succinate dehydrogenase hydrophobic membrane anchor subunit


Mass: 12874.438 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / Strain: K-12 / References: UniProt: I2WBK2

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Non-polymers , 8 types, 24 molecules

#5: Chemical ChemComp-FAD / FLAVIN-ADENINE DINUCLEOTIDE / Flavin adenine dinucleotide


Mass: 785.550 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C27H33N9O15P2 / Comment: FAD*YM
#6: Chemical ChemComp-NA / SODIUM ION / Sodium


Mass: 22.990 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Na
#7: Chemical ChemComp-FES / FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster


Mass: 175.820 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Fe2S2
#8: Chemical ChemComp-SF4 / IRON/SULFUR CLUSTER / Iron–sulfur cluster


Mass: 351.640 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Fe4S4
#9: Chemical ChemComp-F3S / FE3-S4 CLUSTER / Iron–sulfur cluster


Mass: 295.795 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Fe3S4 / Feature type: SUBJECT OF INVESTIGATION
#10: Chemical ChemComp-3PE / 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE / 3-SN-PHOSPHATIDYLETHANOLAMINE / Phosphatidylethanolamine


Mass: 748.065 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C41H82NO8P / Comment: phospholipid*YM
#11: Chemical ChemComp-HEM / PROTOPORPHYRIN IX CONTAINING FE / HEME / Heme B


Mass: 616.487 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Formula: C34H32FeN4O4
#12: Chemical ChemComp-UQ2 / UBIQUINONE-2


Mass: 318.407 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C19H26O4

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Succinate: quinone oxidoreductase SQR from E.coli K12 / Type: COMPLEX / Entity ID: #1-#4 / Source: NATURAL
Source (natural)Organism: Escherichia coli (E. coli) / Strain: K-12
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 40 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 38471 / Symmetry type: POINT

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