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- EMDB-22520: The N-terminus of varicella-zoster virus glycoprotein B has a fun... -

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Entry
Database: EMDB / ID: EMD-22520
TitleThe N-terminus of varicella-zoster virus glycoprotein B has a functional role in fusion
Map data
SampleMonoclonal antibody SG2 bound to varicella-zoster virus glycoprotein B:
varicella-zoster virus glycoprotein B / monoclonal antibody SG2
Biological speciesHuman alphaherpesvirus 3 (Varicella-zoster virus) / unidentified (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 9 Å
AuthorsOliver SL
Funding support United States, 6 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)P41-GM103832 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01-AI102546 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM079429 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R37-AI20459 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI10254601 United States
National Institutes of Health/Office of the DirectorS10-OD021600 United States
CitationJournal: PLoS Pathog / Year: 2021
Title: The N-terminus of varicella-zoster virus glycoprotein B has a functional role in fusion.
Authors: Stefan L Oliver / Yi Xing / Dong-Hua Chen / Soung Hun Roh / Grigore D Pintilie / David A Bushnell / Marvin H Sommer / Edward Yang / Andrea Carfi / Wah Chiu / Ann M Arvin /
Abstract: Varicella-zoster virus (VZV) is a medically important alphaherpesvirus that induces fusion of the virion envelope and the cell membrane during entry, and between cells to form polykaryocytes within ...Varicella-zoster virus (VZV) is a medically important alphaherpesvirus that induces fusion of the virion envelope and the cell membrane during entry, and between cells to form polykaryocytes within infected tissues during pathogenesis. All members of the Herpesviridae, including VZV, have a conserved core fusion complex composed of glycoproteins, gB, gH and gL. The ectodomain of the primary fusogen, gB, has five domains, DI-V, of which DI contains the fusion loops needed for fusion function. We recently demonstrated that DIV is critical for fusion initiation, which was revealed by a 2.8Å structure of a VZV neutralizing mAb, 93k, bound to gB and mutagenesis of the gB-93k interface. To further assess the mechanism of mAb 93k neutralization, the binding site of a non-neutralizing mAb to gB, SG2, was compared to mAb 93k using single particle cryogenic electron microscopy (cryo-EM). The gB-SG2 interface partially overlapped with that of gB-93k but, unlike mAb 93k, mAb SG2 did not interact with the gB N-terminus, suggesting a potential role for the gB N-terminus in membrane fusion. The gB ectodomain structure in the absence of antibody was defined at near atomic resolution by single particle cryo-EM (3.9Å) of native, full-length gB purified from infected cells and by X-ray crystallography (2.4Å) of the transiently expressed ectodomain. Both structures revealed that the VZV gB N-terminus (aa72-114) was flexible based on the absence of visible structures in the cryo-EM or X-ray crystallography data but the presence of gB N-terminal peptides were confirmed by mass spectrometry. Notably, N-terminal residues 109KSQD112 were predicted to form a small α-helix and alanine substitution of these residues abolished cell-cell fusion in a virus-free assay. Importantly, transferring the 109AAAA112 mutation into the VZV genome significantly impaired viral propagation. These data establish a functional role for the gB N-terminus in membrane fusion broadly relevant to the Herpesviridae.
Validation ReportSummary, Full report, XML, About validation report
History
DepositionAug 27, 2020-
Header (metadata) releaseJan 20, 2021-
Map releaseJan 20, 2021-
UpdateJan 20, 2021-
Current statusJan 20, 2021Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 2.16
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 2.16
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_22520.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.57 Å/pix.
x 200 pix.
= 513.2 Å
2.57 Å/pix.
x 200 pix.
= 513.2 Å
2.57 Å/pix.
x 200 pix.
= 513.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.566 Å
Density
Contour LevelBy AUTHOR: 2.16 / Movie #1: 2.16
Minimum - Maximum-7.052689 - 11.431759
Average (Standard dev.)1.0480158e-11 (±0.27297348)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions200200200
Spacing200200200
CellA=B=C: 513.20026 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.5662.5662.566
M x/y/z200200200
origin x/y/z0.0000.0000.000
length x/y/z513.200513.200513.200
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ512512512
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS200200200
D min/max/mean-7.05311.4320.000

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Supplemental data

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Sample components

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Entire Monoclonal antibody SG2 bound to varicella-zoster virus glycoprotein B

EntireName: Monoclonal antibody SG2 bound to varicella-zoster virus glycoprotein B
Number of components: 4

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Component #1: protein, Monoclonal antibody SG2 bound to varicella-zoster virus ...

ProteinName: Monoclonal antibody SG2 bound to varicella-zoster virus glycoprotein B
Recombinant expression: No

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Component #2: protein, varicella-zoster virus glycoprotein B

ProteinName: varicella-zoster virus glycoprotein B / Recombinant expression: No
SourceSpecies: Human alphaherpesvirus 3 (Varicella-zoster virus)

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Component #3: protein, monoclonal antibody SG2

ProteinName: monoclonal antibody SG2 / Recombinant expression: No
SourceSpecies: unidentified (others)

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Component #4: protein, Monoclonal antibody SG2 bound to varicella-zoster virus ...

ProteinName: Monoclonal antibody SG2 bound to varicella-zoster virus glycoprotein B
Recombinant expression: No
SourceSpecies: Human alphaherpesvirus 3 (Varicella-zoster virus)

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Experimental details

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Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7.4
Support filmunspecified
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 7.8 e/Å2 / Illumination mode: OTHER
LensImaging mode: OTHER
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 59610
3D reconstructionResolution: 9 Å / Resolution method: FSC 0.143 CUT-OFF

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