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- EMDB-11288: SARS-CoV-2 Nsp1 bound to the human CCDC124-80S-EBP1 ribosome complex -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-11288
TitleSARS-CoV-2 Nsp1 bound to the human CCDC124-80S-EBP1 ribosome complex
Map data
SampleSARS-CoV-2 Nsp1 bound to the human 80S-CCDC124 ribosome complex
  • human 80S-CCDC124-EBP1 ribosome complex
  • SARS-CoV-2 Nsp1
  • (nucleic-acidNucleic acid) x 5
  • (60S ribosomal protein ...) x 43
  • Ubiquitin-60S ribosomal protein L40
  • 60S acidic ribosomal protein P0
  • (40S ribosomal protein ...) x 31
  • Receptor of activated protein C kinase 1
  • Ubiquitin-40S ribosomal protein S27a
  • Proliferation-associated protein 2G4
  • Coiled-coil domain-containing protein 124
  • Non-structural protein 1
  • (ligand) x 2
Function / homology
Function and homology information


regulation of translation at presynapse, modulating synaptic transmission / negative regulation of formation of translation preinitiation complex / eukaryotic 80S initiation complex / negative regulation of protein neddylation / optic nerve development / TORC2 complex binding / positive regulation of signal transduction by p53 class mediator / response to antineoplastic agent / axial mesoderm development / ribosomal protein import into nucleus ...regulation of translation at presynapse, modulating synaptic transmission / negative regulation of formation of translation preinitiation complex / eukaryotic 80S initiation complex / negative regulation of protein neddylation / optic nerve development / TORC2 complex binding / positive regulation of signal transduction by p53 class mediator / response to antineoplastic agent / axial mesoderm development / ribosomal protein import into nucleus / protein-DNA complex disassembly / 90S preribosome assembly / GAIT complex / response to TNF agonist / negative regulation of DNA repair / oxidized pyrimidine DNA binding / positive regulation of base-excision repair / positive regulation of respiratory burst involved in inflammatory response / positive regulation of DNA N-glycosylase activity / nucleolus organization / protein tyrosine kinase inhibitor activity / positive regulation of gastrulation / positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of Golgi to plasma membrane protein transport / NF-kappaB complex / exit from mitosis / negative regulation of RNA splicing / IRE1-RACK1-PP2A complex / response to extracellular stimulus / ubiquitin ligase inhibitor activity / laminin receptor activity / cytoplasmic side of rough endoplasmic reticulum membrane / retinal ganglion cell axon guidance / middle ear morphogenesis / regulation of cell division / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / positive regulation of endodeoxyribonuclease activity / oxidized purine DNA binding / negative regulation of endoplasmic reticulum unfolded protein response / protein kinase A binding / alpha-beta T cell differentiation / A band / negative regulation of hydrogen peroxide-induced neuron death / supercoiled DNA binding / negative regulation of ubiquitin protein ligase activity / erythrocyte homeostasis / positive regulation of ceramide biosynthetic process / mammalian oogenesis stage / negative regulation of phagocytosis / ubiquitin-like protein conjugating enzyme binding / translation regulator activity / regulation of establishment of cell polarity / signaling adaptor activity / cysteine-type endopeptidase activator activity involved in apoptotic process / negative regulation of Wnt signaling pathway / cytosolic ribosome / activation-induced cell death of T cells / positive regulation of T cell receptor signaling pathway / positive regulation of mitochondrial depolarization / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / negative regulation of smoothened signaling pathway / phagocytic cup / blastocyst development / ion channel inhibitor activity / ribonucleoprotein complex assembly / iron-sulfur cluster binding / positive regulation of activated T cell proliferation / positive regulation of cellular component movement / fibroblast growth factor binding / rescue of stalled ribosome / bone development / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / positive regulation of apoptotic signaling pathway / stress granule assembly / poly(U) RNA binding / monocyte chemotaxis / DNA-(apurinic or apyrimidinic site) lyase / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / positive regulation of ubiquitin-protein transferase activity / BH3 domain binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / erythrocyte development / protein localization to nucleus / positive regulation of cyclic-nucleotide phosphodiesterase activity / spindle assembly / TOR signaling / ribosomal small subunit export from nucleus / cellular response to actinomycin D / regulation of tumor necrosis factor-mediated signaling pathway / positive regulation of JUN kinase activity / maturation of SSU-rRNA / regulation of translational fidelity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / negative regulation of ubiquitin-dependent protein catabolic process / negative regulation of protein kinase B signaling / negative regulation of respiratory burst involved in inflammatory response
Ribosomal protein L15e / Ribosomal protein L11/L12 / Ribosomal protein L10e / Ribosomal protein L15, conserved site / Ribosomal protein L21e / Ribosomal protein L27e / Ribosomal protein L22/L17 / Ribosomal protein S8e / Ribosomal protein L23/L25, conserved site / Peptidase M24 ...Ribosomal protein L15e / Ribosomal protein L11/L12 / Ribosomal protein L10e / Ribosomal protein L15, conserved site / Ribosomal protein L21e / Ribosomal protein L27e / Ribosomal protein L22/L17 / Ribosomal protein S8e / Ribosomal protein L23/L25, conserved site / Peptidase M24 / Ribosomal protein L24e-related / 60S ribosomal protein L6E / Ribosomal protein S26e / Ribosomal protein S14 / Ribosomal protein S4e / Ribosomal protein S5 / Ribosomal protein S9 / Ribosomal protein L6 / Ribosomal protein S8 / Ubiquitin-like domain / Ribosomal protein L3 / Ribosomal protein S27e / Ribosomal protein S15 / Ribosomal protein S7e / Ribosomal protein L44e / RNA-directed RNA polymerase, C-terminal domain / Ribosomal protein S17e / Ribosomal protein L36e / Ribosomal protein S13 / Ribosomal protein S19/S15 / Ribosomal protein L2 / Ribosomal protein L1 / Ribosomal protein L4/L1e / Ribosomal protein L5 / Ribosomal protein S24e / Ribosomal protein L40e / Ribosomal protein S11 / Ribosomal protein S21e / Ribosomal protein L7A/L8 / Ribosomal protein S4/S9, N-terminal / Ribosomal protein S2 / Ribosomal protein S19e / Ribosomal protein L29/L35 / Ribosomal protein S10 / Ribosomal protein L10P / Ribosomal protein L35A / WD40 repeat / Ribosomal protein S3Ae / Ribosomal protein L37e / Ribosomal protein L32e / Ribosomal protein L13e / Ribosomal protein S6e / Ribosomal protein S3, C-terminal / Ribosomal protein S12e / Ribosomal protein S17, conserved site / Macro domain / Ribosomal protein L4 domain superfamily / Ribosomal protein L2, C-terminal / Ribosomal protein L14P, conserved site / DPUP/SUD, C-terminal, betacoronavirus / Ribosomal protein S4/S9 / Ribosomal protein L5 domain superfamily / Ribosomal protein L30e, conserved site / Ribosomal protein S15P / Ribosomal protein L30, central domain, archaeal type / Ribosomal protein L24e, conserved site / Ribosomal protein L13, conserved site / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein S19, superfamily / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein L31e domain superfamily / Ribosomal protein L39e domain superfamily / Ribosomal protein L19/L19e conserved site / Ribosomal protein L2, archaeal-type / Ribosomal protein L1, conserved site / Ribosomal protein L1-like / Ribosomal protein S7 domain / Non-structural protein NSP3, N-terminal, betacoronavirus / Non-structural protein NSP3, SUD-M domain, betacoronavirus / Ribosomal protein L15e core domain superfamily / Ribosomal Proteins L2, RNA binding domain / Non-structural protein NSP1, betacoronavirus / Ribosomal protein S28e / Ribosomal protein L39e, conserved site / Ribosomal protein S17/S11 / Ribosomal protein S5/S7 / Ribosomal protein L30e / Ribosomal protein L14P / Ribosomal protein L19/L19e / Ribosomal protein L39e / Ribosomal protein L31e / Ribosomal protein L18e / Ribosomal protein L23 / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L31e, conserved site / G-protein beta WD-40 repeat / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S5 domain 2-type fold / Ribosomal protein S9, conserved site
60S ribosomal protein L23 / 60S ribosomal protein L30 / 40S ribosomal protein S30 / 40S ribosomal protein S28 / 40S ribosomal protein S26 / 40S ribosomal protein S25 / 40S ribosomal protein S24 / 40S ribosomal protein S15 / 40S ribosomal protein S11 / 40S ribosomal protein S6 ...60S ribosomal protein L23 / 60S ribosomal protein L30 / 40S ribosomal protein S30 / 40S ribosomal protein S28 / 40S ribosomal protein S26 / 40S ribosomal protein S25 / 40S ribosomal protein S24 / 40S ribosomal protein S15 / 40S ribosomal protein S11 / 40S ribosomal protein S6 / 60S ribosomal protein L23a / 40S ribosomal protein S4, X isoform / 60S ribosomal protein L7a / 60S ribosomal protein L31 / 40S ribosomal protein S13 / 40S ribosomal protein S29 / 40S ribosomal protein S18 / 60S ribosomal protein L39 / Ubiquitin-40S ribosomal protein S27a / 60S ribosomal protein L10a / 60S ribosomal protein L36a / Proliferation-associated protein 2G4 / 60S ribosomal protein L10-like / Coiled-coil domain-containing protein 124 / 60S ribosomal protein L18 / 60S ribosomal protein L6 / 60S ribosomal protein L18a / 60S ribosomal protein L19 / 60S ribosomal protein L24 / 60S ribosomal protein L32 / Receptor of activated protein C kinase 1 / 40S ribosomal protein S21 / 60S ribosomal protein L38 / Ubiquitin-60S ribosomal protein L40 / 40S ribosomal protein S14 / 60S ribosomal protein L41 / 60S ribosomal protein L8 / 60S ribosomal protein L11 / 40S ribosomal protein S23 / Replicase polyprotein 1ab / 40S ribosomal protein S16 / 60S ribosomal protein L13 / 60S ribosomal protein L13a / 60S ribosomal protein L3 / 40S ribosomal protein S19 / 60S ribosomal protein L4 / 60S ribosomal protein L22 / 60S ribosomal protein L9 / 60S ribosomal protein L12 / 40S ribosomal protein S12 / 60S ribosomal protein L35 / 40S ribosomal protein S3 / 60S ribosomal protein L17 / 60S ribosomal protein L7 / 60S ribosomal protein L35a / 40S ribosomal protein S2 / 40S ribosomal protein SA / 40S ribosomal protein S17 / 60S acidic ribosomal protein P0 / 40S ribosomal protein S27 / 60S ribosomal protein L27a / 40S ribosomal protein S15a / 40S ribosomal protein S3a / 40S ribosomal protein S8 / 40S ribosomal protein S7 / 60S ribosomal protein L37 / 60S ribosomal protein L37a / 60S ribosomal protein L27 / 60S ribosomal protein L15 / 60S ribosomal protein L26 / 40S ribosomal protein S20 / 60S ribosomal protein L5 / 60S ribosomal protein L14 / 60S ribosomal protein L34 / 60S ribosomal protein L29 / 40S ribosomal protein S10 / 40S ribosomal protein S5 / 40S ribosomal protein S9 / 60S ribosomal protein L28 / 60S ribosomal protein L21 / 60S ribosomal protein L36
Biological speciesHomo sapiens (human) / Severe acute respiratory syndrome coronavirus 2 / Human (human) / 2019-nCoV (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsThoms M / Buschauer R / Ameismeier M / Denk T / Kratzat H / Mackens-Kiani T / Cheng J / Berninghausen O / Becker T / Beckmann R
Funding support Germany, 3 items
OrganizationGrant numberCountry
German Research Foundation (DFG)SFB/TRR-174 Germany
German Research Foundation (DFG)BE1814/1-1 Germany
German Research Foundation (DFG)BE1814/15-1 Germany
CitationJournal: Science / Year: 2020
Title: Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2.
Authors: Matthias Thoms / Robert Buschauer / Michael Ameismeier / Lennart Koepke / Timo Denk / Maximilian Hirschenberger / Hanna Kratzat / Manuel Hayn / Timur Mackens-Kiani / Jingdong Cheng / Jan H ...Authors: Matthias Thoms / Robert Buschauer / Michael Ameismeier / Lennart Koepke / Timo Denk / Maximilian Hirschenberger / Hanna Kratzat / Manuel Hayn / Timur Mackens-Kiani / Jingdong Cheng / Jan H Straub / Christina M Stürzel / Thomas Fröhlich / Otto Berninghausen / Thomas Becker / Frank Kirchhoff / Konstantin M J Sparrer / Roland Beckmann /
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the current coronavirus disease 2019 (COVID-19) pandemic. A major virulence factor of SARS-CoVs is the ...Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the current coronavirus disease 2019 (COVID-19) pandemic. A major virulence factor of SARS-CoVs is the nonstructural protein 1 (Nsp1), which suppresses host gene expression by ribosome association. Here, we show that Nsp1 from SARS-CoV-2 binds to the 40 ribosomal subunit, resulting in shutdown of messenger RNA (mRNA) translation both in vitro and in cells. Structural analysis by cryo-electron microscopy of in vitro-reconstituted Nsp1-40 and various native Nsp1-40 and -80 complexes revealed that the Nsp1 C terminus binds to and obstructs the mRNA entry tunnel. Thereby, Nsp1 effectively blocks retinoic acid-inducible gene I-dependent innate immune responses that would otherwise facilitate clearance of the infection. Thus, the structural characterization of the inhibitory mechanism of Nsp1 may aid structure-based drug design against SARS-CoV-2.
Validation ReportSummary, Full report, XML, About validation report
History
DepositionJul 1, 2020-
Header (metadata) releaseJul 29, 2020-
Map releaseJul 29, 2020-
UpdateSep 16, 2020-
Current statusSep 16, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.06
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.06
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6zm7
  • Surface level: 0.06
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_11288.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 400 pix.
= 423.6 Å
1.06 Å/pix.
x 400 pix.
= 423.6 Å
1.06 Å/pix.
x 400 pix.
= 423.6 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.059 Å
Density
Contour LevelBy AUTHOR: 0.06 / Movie #1: 0.06
Minimum - Maximum-0.12378546 - 0.52678555
Average (Standard dev.)0.0033886929 (±0.01894504)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 423.6 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0591.0591.059
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z423.600423.600423.600
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ400400400
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.1240.5270.003

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Supplemental data

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Sample components

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Entire SARS-CoV-2 Nsp1 bound to the human 80S-CCDC124 ribosome complex

EntireName: SARS-CoV-2 Nsp1 bound to the human 80S-CCDC124 ribosome complex
Number of components: 91

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Component #1: protein, SARS-CoV-2 Nsp1 bound to the human 80S-CCDC124 ribosome ...

ProteinName: SARS-CoV-2 Nsp1 bound to the human 80S-CCDC124 ribosome complex
Recombinant expression: No

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Component #2: protein, human 80S-CCDC124-EBP1 ribosome complex

ProteinName: human 80S-CCDC124-EBP1 ribosome complex / Recombinant expression: No
SourceSpecies: Homo sapiens (human)

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Component #3: protein, SARS-CoV-2 Nsp1

ProteinName: SARS-CoV-2 Nsp1 / Recombinant expression: No
SourceSpecies: Severe acute respiratory syndrome coronavirus 2

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Component #4: nucleic-acid, 28S ribosomal RNA

nucleic acidName: 28S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence: CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAACG GCGAGUGAAC AGGGAAGAGC CCAGCGCCGA AUCCCCGCCC CGCGGCGGGG CGCGGGACAU GUGGCGUACG GAAGACCCGC ...Sequence:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAACG GCGAGUGAAC AGGGAAGAGC CCAGCGCCGA AUCCCCGCCC CGCGGCGGGG CGCGGGACAU GUGGCGUACG GAAGACCCGC UCCCCGGCGC CGCUCGUGGG GGGCCCAAGU CCUUCUGAUC GAGGCCCAGC CCGUGGACGG UGUGAGGCCG GUAGCGGCCC CCGGCGCGCC GGGCCCGGGU CUUCCCGGAG UCGGGUUGCU UGGGAAUGCA GCCCAAAGCG GGUGGUAAAC UCCAUCUAAG GCUAAAUACC GGCACGAGAC CGAUAGUCAA CAAGUACCGU AAGGGAAAGU UGAAAAGAAC UUUGAAGAGA GAGUUCAAGA GGGCGUGAAA CCGUUAAGAG GUAAACGGGU GGGGUCCGCG CAGUCCGCCC GGAGGAUUCA ACCCGGCGGC GGGUCCGGCC GUGUCGGCGG CCCGGCGGAU CUUUCCCGCC CCCCGUUCCU CCCGACCCCU CCACCCGCCC UCCCUUCCCC CGCCGCCCCU CCUCCUCCUC CCCGGAGGGG GCGGGCUCCG GCGGGUGCGG GGGUGGGCGG GCGGGGCCGG GGGUGGGGUC GGCGGGGGAC CGUCCCCCGA CCGGCGACCG GCCGCCGCCG GGCGCAUUUC CACCGCGGCG GUGCGCCGCG ACCGGCUCCG GGACGGCUGG GAAGGCCCGG CGGGGAAGGU GGCUCGGGGG GCCCCGUCCG UCCGUCCGUC CGUCCUCCUC CUCCCCCGUC UCCGCCCCCC GGCCCCGCGU CCUCCCUCGG GAGGGCGCGC GGGUCGGGGC GGCGGCGGCG GCGGCGGUGG CGGCGGCGGC GGCGGCGGCG GGACCGAAAC CCCCCCCGAG UGUUACAGCC CCCCCGGCAG CAGCACUCGC CGAAUCCCGG GGCCGAGGGA GCGAGACCCG UCGCCGCGCU CUCCCCCCUC CCGGCGCCCA CCCCCGCGGG GAAUCCCCCG CGAGGGGGGU CUCCCCCGCG GGGGCGCGCC GGCGUCUCCU CGUGGGGGGG CCGGGCCACC CCUCCCACGG CGCGACCGCU CUCCCACCCC UCCUCCCCGC GCCCCCGCCC CGGCGACGGG GGGGGUGCCG CGCGCGGGUC GGGGGGCGGG GCGGACUGUC CCCAGUGCGC CCCGGGCGGG UCGCGCCGUC GGGCCCGGGG GAGGUUCUCU CGGGGCCACG CGCGCGUCCC CCGAAGAGGG GGACGGCGGA GCGAGCGCAC GGGGUCGGCG GCGACGUCGG CUACCCACCC GACCCGUCUU GAAACACGGA CCAAGGAGUC UAACACGUGC GCGAGUCGGG GGCUCGCACG AAAGCCGCCG UGGCGCAAUG AAGGUGAAGG CCGGCGCGCU CGCCGGCCGA GGUGGGAUCC CGAGGCCUCU CCAGUCCGCC GAGGGCGCAC CACCGGCCCG UCUCGCCCGC CGCGCCGGGG AGGUGGAGCA CGAGCGCACG UGUUAGGACC CGAAAGAUGG UGAACUAUGC CUGGGCAGGG CGAAGCCAGA GGAAACUCUG GUGGAGGUCC GUAGCGGUCC UGACGUGCAA AUCGGUCGUC CGACCUGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGUU CCCUCCGAAG UUUCCCUCAG GAUAGCUGGC GCUCUCGCAG ACCCGACGCA CCCCCGCCAC GCAGUUUUAU CCGGUAAAGC GAAUGAUUAG AGGUCUUGGG GCCGAAACGA UCUCAACCUA UUCUCAAACU UUAAAUGGGU AAGAAGCCCG GCUCGCUGGC GUGGAGCCGG GCGUGGAAUG CGAGUGCCUA GUGGGCCACU UUUGGUAAGC AGAACUGGCG CUGCGGGAUG AACCGAACGC CGGGUUAAGG CGCCCGAUGC CGACGCUCAU CAGACCCCAG AAAAGGUGUU GGUUGAUAUA GACAGCAGGA CGGUGGCCAU GGAAGUCGGA AUCCGCUAAG GAGUGUGUAA CAACUCACCU GCCGAAUCAA CUAGCCCUGA AAAUGGAUGG CGCUGGAGCG UCGGGCCCAU ACCCGGCCGU CGCCGGCAGU CGAGAGUGGA CGGGAGCGGC GGGGGCGGCG CGCGCGCGCG CGCGUGUGGU GUGCGUCGGA GGGCGGCGGC GGCGGCGGCG GCGGGGGUGU GGGGUCCUUC CCCCGCCCCC CCCCCCACGC CUCCUCCCCU CCUCCCGCCC ACGCCCCGCU CCCCGCCCCC GGAGCCCCGC GGACGCUACG CCGCGACGAG UAGGAGGGCC GCUGCGGUGA GCCUUGAAGC CUAGGGCGCG GGCCCGGGUG GAGCCGCCGC AGGUGCAGAU CUUGGUGGUA GUAGCAAAUA UUCAAACGAG AACUUUGAAG GCCGAAGUGG AGAAGGGUUC CAUGUGAACA GCAGUUGAAC AUGGGUCAGU CGGUCCUGAG AGAUGGGCGA GCGCCGUUCC GAAGGGACGG GCGAUGGCCU CCGUUGCCCU CGGCCGAUCG AAAGGGAGUC GGGUUCAGAU CCCCGAAUCC GGAGUGGCGG AGAUGGGCGC CGCGAGGCGU CCAGUGCGGU AACGCGACCG AUCCCGGAGA AGCCGGCGGG AGCCCCGGGG AGAGUUCUCU UUUCUUUGUG AAGGGCAGGG CGCCCUGGAA UGGGUUCGCC CCGAGAGAGG GGCCCGUGCC UUGGAAAGCG UCGCGGUUCC GGCGGCGUCC GGUGAGCUCU CGCUGGCCCU UGAAAAUCCG GGGGAGAGGG UGUAAAUCUC GCGCCGGGCC GUACCCAUAU CCGCAGCAGG UCUCCAAGGU GAACAGCCUC UGGCAUGUUG GAACAAUGUA GGUAAGGGAA GUCGGCAAGC CGGAUCCGUA ACUUCGGGAU AAGGAUUGGC UCUAAGGGCU GGGUCGGUCG GGCUGGGGCG CGAAGCGGGG CUGGGCGCGC GCCGCGGCUG GACGAGGCGC CGCCGCCCCC CCCACGCCCG GGGCACCCCC CUCGCGGCCC UCCCCCGCCC CACCCCGCGC GCGCCGCUCG CUCCCUCCCC GCCCCGCGCC CUCUCUCUCU CUCUCUCCCC CGCUCCCCGU CCUCCCCCCU CCCCGGGGGA GCGCCGCGUG GGGGCGGCGG CGGGGGGAGA AGGGUCGGGG CGGCAGGGGC CGGCGGCGGC CCGCCGCGGG GCCCCGGCGG CGGGGGCACG GUCCCCCGCG AGGGGGGCCC GGGCACCCGG GGGGCCGGCG GCGGCGGCGA CUCUGGACGC GAGCCGGGCC CUUCCCGUGG AUCGCCCCAG CUGCGGCGGG CGUCGCGGCC GCCCCCGGGG AGCCCGGCGG GCGCCGGCGC GCCCCCCCCC CCACCCCACG UCUCGUCGCG CGCGCGUCCG CUGGGGGCGG GGAGCGGUCG GGCGGCGGCG GUCGGCGGGC GGCGGGGCGG GGCGGUUCGU CCCCCCGCCC UACCCCCCCG GCCCCGUCCG CCCCCCGUUC CCCCCUCCUC CUCGGCGCGC GGCGGCGGCG GCGGGCGGCG GAGGGGCCGC GGGCCGGUCC CCCCCGCCGG GUCCGCCCCC GGGGCCGCGG UUCCGCGCGG CGCCUCGCCU CGGCCGGCGC CUAGCAGCCG ACUUAGAACU GGUGCGGACC AGGGGAAUCC GACUGUUUAA UUAAAACAAA GCAUCGCGAA GGCCCGCGGC GGGUGUUGAC GCGAUGUGAU UUCUGCCCAG UGCUCUGAAU GUCAAAGUGA AGAAAUUCAA UGAAGCGCGG GUAAACGGCG GGAGUAACUA UGACUCUCUU AAGGUAGCCA AAUGCCUCGU CAUCUAAUUA GUGACGCGCA UGAAUGGAUG AACGAGAUUC CCACUGUCCC UACCUACUAU CCAGCGAAAC CACAGCCAAG GGAACGGGCU UGGCGGAAUC AGCGGGGAAA GAAGACCCUG UUGAGCUUGA CUCUAGUCUG GCACGGUGAA GAGACAUGAG AGGUGUAGAA UAAGUGGGAG GCCCCCGGCG CCCCCCCGGU GUCCCCGCGA GGGGCCCGGG GCGGGGUCCG CCGGCCCUGC GGGCCGCCGG UGAAAUACCA CUACUCUGAU CGUUUUUUCA CUGACCCGGU GAGGCGGGGG GGCGAGCCCC GAGGGGCUCU CGCUUCUGGC GCCAAGCGCC CGGCCGCGCG CCGGCCGGGC GCGACCCGCU CCGGGGACAG UGCCAGGUGG GGAGUUUGAC UGGGGCGGUA CACCUGUCAA ACGGUAACGC AGGUGUCCUA AGGCGAGCUC AGGGAGGACA GAAACCUCCC GUGGAGCAGA AGGGCAAAAG CUCGCUUGAU CUUGAUUUUC AGUACGAAUA CAGACCGUGA AAGCGGGGCC UCACGAUCCU UCUGACCUUU UGGGUUUUAA GCAGGAGGUG UCAGAAAAGU UACCACAGGG AUAACUGGCU UGUGGCGGCC AAGCGUUCAU AGCGACGUCG CUUUUUGAUC CUUCGAUGUC GGCUCUUCCU AUCAUUGUGA AGCAGAAUUC ACCAAGCGUU GGAUUGUUCA CCCACUAAUA GGGAACGUGA GCUGGGUUUA GACCGUCGUG AGACAGGUUA GUUUUACCCU ACUGAUGAUG UGUUGUUGCC AUGGUAAUCC UGCUCAGUAC GAGAGGAACC GCAGGUUCAG ACAUUUGGUG UAUGUGCUUG GCUGAGGAGC CAAUGGGGCG AAGCUACCAU CUGUGGGAUU AUGACUGAAC GCCUCUAAGU CAGAAUCCCG CCCAGGCGGA ACGAUACGGC AGCGCCGCGG AGCCUCGGUU GGCCUCGGAU AGCCGGUCCC CCGCCUGUCC CCGCCGGCGG GCCGCCCCCC CCUCCACGCG CCCCGCGCGC GCGGGAGGGC GCGUGCCCCG CCGCGCGCCG GGACCGGGGU CCGGUGCGGA GUGCCCUUCG UCCUGGGAAA CGGGGCGCGG CUGGAAAGGC GGCCGCCCCC UCGCCCGUCA CGCACCGCAC GUUCGUGGGG AACCUGGCGC UAAACCAUUC GUAGACGACC UGCUUCUGGG UCGGGGUUUC GUACGUAGCA GAGCAGCUCC CUCGCUGCGA UCUAUUGAAA GUCAGCCCUC GACACAAGGG UUUGUC
MassTheoretical: 1.638922 MDa
SourceSpecies: Human (human)

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Component #5: nucleic-acid, 5S ribosomal RNA

nucleic acidName: 5S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAGA CCGCCUGGGA AUACCGGGUG CUGUAGGCUU U
MassTheoretical: 38.998078 kDa
SourceSpecies: Human (human)

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Component #6: nucleic-acid, 5.8S ribosomal RNA

nucleic acidName: 5.8S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAUC AUCGACACUU CGAACGCACU UGCGGCCCCG GGUUCCUCCC GGGGCUACGC CUGUCUGAGC GUCGCUU
MassTheoretical: 50.449812 kDa
SourceSpecies: Human (human)

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Component #7: protein, 60S ribosomal protein L8

ProteinName: 60S ribosomal protein L8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.088863 kDa
SourceSpecies: Human (human)

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Component #8: protein, 60S ribosomal protein L3

ProteinName: 60S ribosomal protein L3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 46.211113 kDa
SourceSpecies: Human (human)

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Component #9: protein, 60S ribosomal protein L4

ProteinName: 60S ribosomal protein L4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 47.804621 kDa
SourceSpecies: Human (human)

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Component #10: protein, 60S ribosomal protein L5

ProteinName: 60S ribosomal protein L5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.426789 kDa
SourceSpecies: Human (human)

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Component #11: protein, 60S ribosomal protein L6

ProteinName: 60S ribosomal protein L6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 32.810176 kDa
SourceSpecies: Human (human)

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Component #12: protein, 60S ribosomal protein L7

ProteinName: 60S ribosomal protein L7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.290973 kDa
SourceSpecies: Human (human)

+
Component #13: protein, 60S ribosomal protein L7a

ProteinName: 60S ribosomal protein L7a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 30.061785 kDa
SourceSpecies: Human (human)

+
Component #14: protein, 60S ribosomal protein L9

ProteinName: 60S ribosomal protein L9 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.899471 kDa
SourceSpecies: Human (human)

+
Component #15: protein, 60S ribosomal protein L10-like

ProteinName: 60S ribosomal protein L10-like / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.570949 kDa
SourceSpecies: Human (human)

+
Component #16: protein, 60S ribosomal protein L11

ProteinName: 60S ribosomal protein L11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.288465 kDa
SourceSpecies: Human (human)

+
Component #17: protein, 60S ribosomal protein L13

ProteinName: 60S ribosomal protein L13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.321682 kDa
SourceSpecies: Human (human)

+
Component #18: protein, 60S ribosomal protein L14

ProteinName: 60S ribosomal protein L14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.485016 kDa
SourceSpecies: Human (human)

+
Component #19: protein, 60S ribosomal protein L15

ProteinName: 60S ribosomal protein L15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.207285 kDa
SourceSpecies: Human (human)

+
Component #20: protein, 60S ribosomal protein L13a

ProteinName: 60S ribosomal protein L13a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.633412 kDa
SourceSpecies: Human (human)

+
Component #21: protein, 60S ribosomal protein L17

ProteinName: 60S ribosomal protein L17 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.44317 kDa
SourceSpecies: Human (human)

+
Component #22: protein, 60S ribosomal protein L18

ProteinName: 60S ribosomal protein L18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.687676 kDa
SourceSpecies: Human (human)

+
Component #23: protein, 60S ribosomal protein L19

ProteinName: 60S ribosomal protein L19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.535281 kDa
SourceSpecies: Human (human)

+
Component #24: protein, 60S ribosomal protein L18a

ProteinName: 60S ribosomal protein L18a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.808514 kDa
SourceSpecies: Human (human)

+
Component #25: protein, 60S ribosomal protein L21

ProteinName: 60S ribosomal protein L21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.609988 kDa
SourceSpecies: Human (human)

+
Component #26: protein, 60S ribosomal protein L22

ProteinName: 60S ribosomal protein L22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.813015 kDa
SourceSpecies: Human (human)

+
Component #27: protein, 60S ribosomal protein L23

ProteinName: 60S ribosomal protein L23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.892505 kDa
SourceSpecies: Human (human)

+
Component #28: protein, 60S ribosomal protein L24

ProteinName: 60S ribosomal protein L24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.825111 kDa
SourceSpecies: Human (human)

+
Component #29: protein, 60S ribosomal protein L23a

ProteinName: 60S ribosomal protein L23a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.740193 kDa
SourceSpecies: Human (human)

+
Component #30: protein, 60S ribosomal protein L26

ProteinName: 60S ribosomal protein L26 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.303363 kDa
SourceSpecies: Human (human)

+
Component #31: protein, 60S ribosomal protein L27

ProteinName: 60S ribosomal protein L27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.835831 kDa
SourceSpecies: Human (human)

+
Component #32: protein, 60S ribosomal protein L27a

ProteinName: 60S ribosomal protein L27a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.604535 kDa
SourceSpecies: Human (human)

+
Component #33: protein, 60S ribosomal protein L29

ProteinName: 60S ribosomal protein L29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.804275 kDa
SourceSpecies: Human (human)

+
Component #34: protein, 60S ribosomal protein L30

ProteinName: 60S ribosomal protein L30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.805092 kDa
SourceSpecies: Human (human)

+
Component #35: protein, 60S ribosomal protein L31

ProteinName: 60S ribosomal protein L31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.494938 kDa
SourceSpecies: Human (human)

+
Component #36: protein, 60S ribosomal protein L32

ProteinName: 60S ribosomal protein L32 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.898932 kDa
SourceSpecies: Human (human)

+
Component #37: protein, 60S ribosomal protein L35a

ProteinName: 60S ribosomal protein L35a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.564743 kDa
SourceSpecies: Human (human)

+
Component #38: protein, 60S ribosomal protein L34

ProteinName: 60S ribosomal protein L34 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.326074 kDa
SourceSpecies: Human (human)

+
Component #39: protein, 60S ribosomal protein L35

ProteinName: 60S ribosomal protein L35 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.593624 kDa
SourceSpecies: Human (human)

+
Component #40: protein, 60S ribosomal protein L36

ProteinName: 60S ribosomal protein L36 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.290859 kDa
SourceSpecies: Human (human)

+
Component #41: protein, 60S ribosomal protein L37

ProteinName: 60S ribosomal protein L37 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.111032 kDa
SourceSpecies: Human (human)

+
Component #42: protein, 60S ribosomal protein L38

ProteinName: 60S ribosomal protein L38 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.238948 kDa
SourceSpecies: Human (human)

+
Component #43: protein, 60S ribosomal protein L39

ProteinName: 60S ribosomal protein L39 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.426759 kDa
SourceSpecies: Human (human)

+
Component #44: protein, Ubiquitin-60S ribosomal protein L40

ProteinName: Ubiquitin-60S ribosomal protein L40 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.758394 kDa
SourceSpecies: Human (human)

+
Component #45: protein, 60S ribosomal protein L41

ProteinName: 60S ribosomal protein L41 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 3.473451 kDa
SourceSpecies: Human (human)

+
Component #46: protein, 60S ribosomal protein L36a

ProteinName: 60S ribosomal protein L36a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.476973 kDa
SourceSpecies: Human (human)

+
Component #47: protein, 60S ribosomal protein L37a

ProteinName: 60S ribosomal protein L37a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.29935 kDa
SourceSpecies: Human (human)

+
Component #48: protein, 60S ribosomal protein L28

ProteinName: 60S ribosomal protein L28 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.784622 kDa
SourceSpecies: Human (human)

+
Component #49: protein, 60S acidic ribosomal protein P0

ProteinName: 60S acidic ribosomal protein P0 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.309418 kDa
SourceSpecies: Human (human)

+
Component #50: protein, 60S ribosomal protein L12

ProteinName: 60S ribosomal protein L12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.847619 kDa
SourceSpecies: Human (human)

+
Component #51: protein, 60S ribosomal protein L10a

ProteinName: 60S ribosomal protein L10a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.879422 kDa
SourceSpecies: Human (human)

+
Component #52: nucleic-acid, 18S ribosomal RNA

nucleic acidName: 18S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence: UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUG AGUACGCACG GCCGGUACAG UGAAACUGCG AAUGGCUCAU UAAAUCAGUU AUGGUUCCUU UGGUCGCUCG CUCCUCUCCU ACUUGGAUAA CUGUGGUAAU UCUAGAGCUA ...Sequence:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUG AGUACGCACG GCCGGUACAG UGAAACUGCG AAUGGCUCAU UAAAUCAGUU AUGGUUCCUU UGGUCGCUCG CUCCUCUCCU ACUUGGAUAA CUGUGGUAAU UCUAGAGCUA AUACAUGCCG ACGGGCGCUG ACCCCCUUCG CGGGGGGGAU GCGUGCAUUU AUCAGAUCAA AACCAACCCG GUCAGCCCCU CUCCGGCCCC GGCCGGGGGG CGGGCGCCGG CGGCUUUGGU GACUCUAGAU AACCUCGGGC CGAUCGCACG CCCCCCGUGG CGGCGACGAC CCAUUCGAAC GUCUGCCCUA UCAACUUUCG AUGGUAGUCG CCGUGCCUAC CAUGGUGACC ACGGGUGACG GGGAAUCAGG GUUCGAUUCC GGAGAGGGAG CCUGAGAAAC GGCUACCACA UCCAAGGAAG GCAGCAGGCG CGCAAAUUAC CCACUCCCGA CCCGGGGAGG UAGUGACGAA AAAUAACAAU ACAGGACUCU UUCGAGGCCC UGUAAUUGGA AUGAGUCCAC UUUAAAUCCU UUAACGAGGA UCCAUUGGAG GGCAAGUCUG GUGCCAGCAG CCGCGGUAAU UCCAGCUCCA AUAGCGUAUA UUAAAGUUGC UGCAGUUAAA AAGCUCGUAG UUGGAUCUUG GGAGCGGGCG GGCGGUCCGC CGCGAGGCGA GCCACCGCCC GUCCCCGCCC CUUGCCUCUC GGCGCCCCCU CGAUGCUCUU AGCUGAGUGU CCCGCGGGGC CCGAAGCGUU UACUUUGAAA AAAUUAGAGU GUUCAAAGCA GGCCCGAGCC GCCUGGAUAC CGCAGCUAGG AAUAAUGGAA UAGGACCGCG GUUCUAUUUU GUUGGUUUUC GGAACUGAGG CCAUGAUUAA GAGGGACGGC CGGGGGCAUU CGUAUUGCGC CGCUAGAGGU GAAAUUCUUG GACCGGCGCA AGACGGACCA GAGCGAAAGC AUUUGCCAAG AAUGUUUUCA UUAAUCAAGA ACGAAAGUCG GAGGUUCGAA GACGAUCAGA UACCGUCGUA GUUCCGACCA UAAACGAUGC CGACCGGCGA UGCGGCGGCG UUAUUCCCAU GACCCGCCGG GCAGCUUCCG GGAAACCAAA GUCUUUGGGU UCCGGGGGGA GUAUGGUUGC AAAGCUGAAA CUUAAAGGAA UUGACGGAAG GGCACCACCA GGAGUGGAGC CUGCGGCUUA AUUUGACUCA ACACGGGAAA CCUCACCCGG CCCGGACACG GACAGGAUUG ACAGAUUGAU AGCUCUUUCU CGAUUCCGUG GGUGGUGGUG CAUGGCCGUU CUUAGUUGGU GGAGCGAUUU GUCUGGUUAA UUCCGAUAAC GAACGAGACU CUGGCAUGCU AACUAGUUAC GCGACCCCCG AGCGGUCGGC GUCCCCCAAC UUCUUAGAGG GACAAGUGGC GUUCAGCCAC CCGAGAUUGA GCAAUAACAG GUCUGUGAUG CCCUUAGAUG UCCGGGGCUG CACGCGCGCU ACACUGACUG GCUCAGCGUG UGCCUACCCU ACGCCGGCAG GCGCGGGUAA CCCGUUGAAC CCCAUUCGUG AUGGGGAUCG GGGAUUGCAA UUAUUCCCCA UGAACGAGGA AUUCCCAGUA AGUGCGGGUC AUAAGCUUGC GUUGAUUAAG UCCCUGCCCU UUGUACACAC CGCCCGUCGC UACUACCGAU UGGAUGGUUU AGUGAGGCCC UCGGAUCGGC CCCGCCGGGG UCGGCCCACG GCCCUGGCGG AGCGCUGAGA AGACGGUCGA ACUUGACUAU CUAGAGGAAG UAAAAGUCGU AACAAGGUUU CCGUAGGUGA ACCUGCGGAA GGAUCAUUA
MassTheoretical: 602.793875 kDa
SourceSpecies: Human (human)

+
Component #53: protein, 40S ribosomal protein SA

ProteinName: 40S ribosomal protein SA / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 32.883938 kDa
SourceSpecies: Human (human)

+
Component #54: protein, 40S ribosomal protein S3a

ProteinName: 40S ribosomal protein S3a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 30.002061 kDa
SourceSpecies: Human (human)

+
Component #55: protein, 40S ribosomal protein S3

ProteinName: 40S ribosomal protein S3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.729369 kDa
SourceSpecies: Human (human)

+
Component #56: protein, 40S ribosomal protein S4, X isoform

ProteinName: 40S ribosomal protein S4, X isoformRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.654869 kDa
SourceSpecies: Human (human)

+
Component #57: protein, 40S ribosomal protein S5

ProteinName: 40S ribosomal protein S5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.913453 kDa
SourceSpecies: Human (human)

+
Component #58: protein, 40S ribosomal protein S7

ProteinName: 40S ribosomal protein S7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.168914 kDa
SourceSpecies: Human (human)

+
Component #59: protein, 40S ribosomal protein S8

ProteinName: 40S ribosomal protein S8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.263387 kDa
SourceSpecies: Human (human)

+
Component #60: protein, 40S ribosomal protein S10

ProteinName: 40S ribosomal protein S10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.933846 kDa
SourceSpecies: Human (human)

+
Component #61: protein, 40S ribosomal protein S11

ProteinName: 40S ribosomal protein S11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.468826 kDa
SourceSpecies: Human (human)

+
Component #62: protein, 40S ribosomal protein S15

ProteinName: 40S ribosomal protein S15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.076207 kDa
SourceSpecies: Human (human)

+
Component #63: protein, 40S ribosomal protein S16

ProteinName: 40S ribosomal protein S16 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.477377 kDa
SourceSpecies: Human (human)

+
Component #64: protein, 40S ribosomal protein S17

ProteinName: 40S ribosomal protein S17 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.578156 kDa
SourceSpecies: Human (human)

+
Component #65: protein, 40S ribosomal protein S18

ProteinName: 40S ribosomal protein S18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.759777 kDa
SourceSpecies: Human (human)

+
Component #66: protein, 40S ribosomal protein S19

ProteinName: 40S ribosomal protein S19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.091562 kDa
SourceSpecies: Human (human)

+
Component #67: protein, 40S ribosomal protein S20

ProteinName: 40S ribosomal protein S20 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.398763 kDa
SourceSpecies: Human (human)

+
Component #68: protein, 40S ribosomal protein S21

ProteinName: 40S ribosomal protein S21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.124389 kDa
SourceSpecies: Human (human)

+
Component #69: protein, 40S ribosomal protein S23

ProteinName: 40S ribosomal protein S23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.844666 kDa
SourceSpecies: Human (human)

+
Component #70: protein, 40S ribosomal protein S26

ProteinName: 40S ribosomal protein S26 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.047532 kDa
SourceSpecies: Human (human)

+
Component #71: protein, 40S ribosomal protein S28

ProteinName: 40S ribosomal protein S28 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.855052 kDa
SourceSpecies: Human (human)

+
Component #72: protein, 40S ribosomal protein S29

ProteinName: 40S ribosomal protein S29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.690821 kDa
SourceSpecies: Human (human)

+
Component #73: protein, Receptor of activated protein C kinase 1

ProteinName: Receptor of activated protein C kinase 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 35.115652 kDa
SourceSpecies: Human (human)

+
Component #74: protein, 40S ribosomal protein S2

ProteinName: 40S ribosomal protein S2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 31.376516 kDa
SourceSpecies: Human (human)

+
Component #75: protein, 40S ribosomal protein S6

ProteinName: 40S ribosomal protein S6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.751906 kDa
SourceSpecies: Human (human)

+
Component #76: protein, 40S ribosomal protein S9

ProteinName: 40S ribosomal protein S9 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.641564 kDa
SourceSpecies: Human (human)

+
Component #77: protein, 40S ribosomal protein S12

ProteinName: 40S ribosomal protein S12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.538987 kDa
SourceSpecies: Human (human)

+
Component #78: protein, 40S ribosomal protein S13

ProteinName: 40S ribosomal protein S13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.259389 kDa
SourceSpecies: Human (human)

+
Component #79: protein, 40S ribosomal protein S14

ProteinName: 40S ribosomal protein S14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.302772 kDa
SourceSpecies: Human (human)

+
Component #80: protein, 40S ribosomal protein S15a

ProteinName: 40S ribosomal protein S15a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.865555 kDa
SourceSpecies: Human (human)

+
Component #81: protein, 40S ribosomal protein S24

ProteinName: 40S ribosomal protein S24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.463333 kDa
SourceSpecies: Human (human)

+
Component #82: protein, 40S ribosomal protein S25

ProteinName: 40S ribosomal protein S25 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.776224 kDa
SourceSpecies: Human (human)

+
Component #83: protein, 40S ribosomal protein S27

ProteinName: 40S ribosomal protein S27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.480186 kDa
SourceSpecies: Human (human)

+
Component #84: protein, 40S ribosomal protein S30

ProteinName: 40S ribosomal protein S30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.668938 kDa
SourceSpecies: Human (human)

+
Component #85: protein, Ubiquitin-40S ribosomal protein S27a

ProteinName: Ubiquitin-40S ribosomal protein S27a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.004041 kDa
SourceSpecies: Human (human)

+
Component #86: protein, Proliferation-associated protein 2G4

ProteinName: Proliferation-associated protein 2G4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 43.851879 kDa
SourceSpecies: Human (human)

+
Component #87: nucleic-acid, tRNA

nucleic acidName: tRNATransfer RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UCCGAUAUAG CGUAACGGCU AUCACAUCAC GCUUUCACCG UGGAGACCGG GGUUCGACUC CCCGUAUCGG AGCCA
MassTheoretical: 24.004262 kDa
SourceSpecies: Human (human)

+
Component #88: protein, Coiled-coil domain-containing protein 124

ProteinName: Coiled-coil domain-containing protein 124 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.890377 kDa
SourceSpecies: Human (human)

+
Component #89: protein, Non-structural protein 1

ProteinName: Non-structural protein 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.801287 kDa
SourceSpecies: 2019-nCoV (virus)

+
Component #90: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 256 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

+
Component #91: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 8 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

-
Experimental details

-
Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 44.8 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

-
Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 52512
3D reconstructionResolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot (resolution estimation)

-
Atomic model buiding

Output model

+
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