[English] 日本語
Yorodumi Papers
- Database of articles cited by EMDB/PDB/SASBDB data -

+
Search query

Keywords
Structure methods
Author
Journal
IF

-
Structure paper

TitleTubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility.
Journal, issue, pagesScience, Vol. 371, Issue 6525, Year 2021
Publish dateJan 8, 2021
AuthorsSudarshan Gadadhar / Gonzalo Alvarez Viar / Jan Niklas Hansen / An Gong / Aleksandr Kostarev / Côme Ialy-Radio / Sophie Leboucher / Marjorie Whitfield / Ahmed Ziyyat / Aminata Touré / Luis Alvarez / Gaia Pigino / Carsten Janke /
PubMed AbstractPosttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. ...Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. Tubulin glycylation modifies microtubules specifically in cilia and flagella, but its functional and mechanistic roles remain unclear. In this study, we generated a mouse model entirely lacking tubulin glycylation. Male mice were subfertile owing to aberrant beat patterns of their sperm flagella, which impeded the straight swimming of sperm cells. Using cryo-electron tomography, we showed that lack of glycylation caused abnormal conformations of the dynein arms within sperm axonemes, providing the structural basis for the observed dysfunction. Our findings reveal the importance of microtubule glycylation for controlled flagellar beating, directional sperm swimming, and male fertility.
External linksScience / PubMed:33414192
MethodsEM (subtomogram averaging)
Resolution24 - 38.4 Å
Structure data

EMDB-11942:
""Tubulin glycylation controls axonemal dynein activity, flagellar beat and male fertility"": Pre-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs). Both beta- and gamma-heavy chains are in pre-power stroke conformation. Cryo-ET, classification and sub-tomogram averaging.
Method: EM (subtomogram averaging) / Resolution: 24.7 Å

EMDB-11943:
""Tubulin glycylation controls axonemal dynein activity, flagellar beat and male fertility"": Pre-post-power stroke conformation of the mouse sperm outer dynein arms (ODAs), with gamma-heavy chain in pre-power stroke conformation and beta-heavy chain in post-power stroke conformation. Cryo-ET, classification and sub-tomogram averaging.
Method: EM (subtomogram averaging) / Resolution: 35.7 Å

EMDB-11944:
Post-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs), with gamma-heavy chain in post-power stroke conformation and beta-heavy chain in pre-power stroke conformation. Reconstruction obtained by sub-tomogram classification and averaging from cryo-electron tomograms of active flagella of mouse sperm cells.
Method: EM (subtomogram averaging) / Resolution: 38.4 Å

EMDB-11945:
Post-post-power stroke conformation of the mouse sperm outer dynein arms (ODAs), with both gamma-heavy chain and beta-heavy chain in post-power stroke conformation. Reconstruction obtained with sub-tomogram classification and averaging from cryo-electron tomograms of active flagella of mouse sperm cells.
Method: EM (subtomogram averaging) / Resolution: 34.5 Å

EMDB-11946:
Axonemal 96nm-repeat from active sperm flagella of wild type mouse. Reconstruction obtained with cryo-ET and sub-tomogram averaging.
Method: EM (subtomogram averaging) / Resolution: 24 Å

EMDB-11947:
Axonemal 96nm-repeat from active sperm flagella of glycylation deficient mouse (Ttll3-/-Ttll8-/- mutation). Reconstruction obtained with cryo-ET and sub-tomogram averaging.
Method: EM (subtomogram averaging) / Resolution: 33 Å

EMDB-11948:
Axonemal 96nm-repeat from active flagella of wild type mouse, showing inner dynein arms (IDAs) in pre-power stroke conformations. Reconstruction obtained with classification and averaging of sub-tomograms from cryo-electron tomograms of wild type active flagella.
Method: EM (subtomogram averaging) / Resolution: 30.7 Å

EMDB-11949:
Axonemal 96nm-repeat from active flagella of wild type mouse, showing inner dynein arms (IDAs) in post-power stroke conformations. Reconstruction obtained with classification and averaging of sub-tomograms from cryo-electron tomograms of wild type active flagella.
Method: EM (subtomogram averaging) / Resolution: 33.2 Å

Source
  • Mus musculus (house mouse)
KeywordsAnimals / Axonemal Dyneins / Cilia / Cryoelectron Microscopy / Disease Models, Animal / Electron Microscope Tomography / Fertility / Infertility, Male / Male / Mice / Mice, Knockout / Protein Processing, Post-Translational / Sperm Motility / Sperm Tail / Tubulin

+
About Yorodumi Papers

-
News

-
Aug 12, 2020. New: Covid-19 info

New: Covid-19 info

  • New page: Covid-19 featured information page in EM Navigator

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

-
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. New: Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242):Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force. (see PDBe EMDB page)
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is "EMD"? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB at PDBe / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary. This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated. See below links for details.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software). Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

+
Jun 16, 2017. Omokage search with filter

Omokage search with filter

  • Result of Omokage search can be filtered by keywords and the database types

Related info.:Omokage search

Read more

-
Yorodumi Papers

Database of articles cited by EMDB/PDB/SASBDB data

  • Database of articles cited by EMDB, PDB, and SASBDB entries
  • Using PubMed data

Related info.:EMDB / PDB / SASBDB / Yorodumi / EMN Papers / Changes in new EM Navigator and Yorodumi

Read more