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TitleCryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 6437, Year 2020
Publish dateDec 22, 2020
AuthorsFlorian Fäßler / Georgi Dimchev / Victor-Valentin Hodirnau / William Wan / Florian K M Schur /
PubMed AbstractThe actin-related protein (Arp)2/3 complex nucleates branched actin filament networks pivotal for cell migration, endocytosis and pathogen infection. Its activation is tightly regulated and involves ...The actin-related protein (Arp)2/3 complex nucleates branched actin filament networks pivotal for cell migration, endocytosis and pathogen infection. Its activation is tightly regulated and involves complex structural rearrangements and actin filament binding, which are yet to be understood. Here, we report a 9.0 Å resolution structure of the actin filament Arp2/3 complex branch junction in cells using cryo-electron tomography and subtomogram averaging. This allows us to generate an accurate model of the active Arp2/3 complex in the branch junction and its interaction with actin filaments. Notably, our model reveals a previously undescribed set of interactions of the Arp2/3 complex with the mother filament, significantly different to the previous branch junction model. Our structure also indicates a central role for the ArpC3 subunit in stabilizing the active conformation.
External linksNat Commun / PubMed:33353942 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution9 Å
Structure data

EMDB-11869: Structure of the actin filament Arp2/3 complex branch junction in cells
PDB-7aqk: Model of the actin filament Arp2/3 complex branch junction in cells
Method: EM (subtomogram averaging) / Resolution: 9 Å

EMDB-11870:
Tomogram of the actin network in an extracted and fixed mouse fibroblast lamellipodium.
Method: EM (tomography)

Source
  • mus musculus (house mouse)
  • House mouse (house mouse)
KeywordsActin Cytoskeleton / Actin-Related Protein 2-3 Complex / Animals / Electron Microscope Tomography / Fibroblasts / Mice / Models, Molecular / NIH 3T3 Cells / Protein Conformation / Pseudopodia / STRUCTURAL PROTEIN / Actin / Arp2-3 complex / Cytoskeleton

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