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TitleStructure of a cyanobacterial photosystem I tetramer revealed by cryo-electron microscopy.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 4929, Year 2019
Publish dateOct 30, 2019
AuthorsKoji Kato / Ryo Nagao / Tian-Yi Jiang / Yoshifumi Ueno / Makio Yokono / Siu Kit Chan / Mai Watanabe / Masahiko Ikeuchi / Jian-Ren Shen / Seiji Akimoto / Naoyuki Miyazaki / Fusamichi Akita /
PubMed AbstractPhotosystem I (PSI) functions to harvest light energy for conversion into chemical energy. The organisation of PSI is variable depending on the species of organism. Here we report the structure of a ...Photosystem I (PSI) functions to harvest light energy for conversion into chemical energy. The organisation of PSI is variable depending on the species of organism. Here we report the structure of a tetrameric PSI core isolated from a cyanobacterium, Anabaena sp. PCC 7120, analysed by single-particle cryo-electron microscopy (cryo-EM) at 3.3 Å resolution. The PSI tetramer has a C2 symmetry and is organised in a dimer of dimers form. The structure reveals interactions at the dimer-dimer interface and the existence of characteristic pigment orientations and inter-pigment distances within the dimer units that are important for unique excitation energy transfer. In particular, characteristic residues of PsaL are identified to be responsible for the formation of the tetramer. Time-resolved fluorescence analyses showed that the PSI tetramer has an enhanced excitation-energy quenching. These structural and spectroscopic findings provide insights into the physiological significance of the PSI tetramer and evolutionary changes of the PSI organisations.
External linksNat Commun / PubMed:31666526 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 4 Å
Structure data

EMDB-9807, PDB-6jeo:
Structure of PSI tetramer from Anabaena
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-9877:
Structure of PSI dimer from Anabaena
Method: EM (single particle) / Resolution: 4 Å

Chemicals

ChemComp-CL0:
CHLOROPHYLL A ISOMER / Chlorophyll a

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-BCR:
BETA-CAROTENE / Beta-Carotene

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

Source
  • Nostoc sp. PCC 7120 (Cyanobacteria)
  • nostoc sp. (strain pcc 7120 / sag 25.82 / utex 2576) (Cyanobacteria)
KeywordsAnabaena / Cryoelectron Microscopy / Photosystem I Protein Complex / Protein Structure, Quaternary / Single Molecule Imaging / Spectrometry, Fluorescence / photosystem I, psaB subunit / photosystem I, psaL subunit / PHOTOSYNTHESIS / Photosystem

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