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TitleConformational switches control early maturation of the eukaryotic small ribosomal subunit.
Journal, issue, pagesElife, Vol. 8, Year 2019
Publish dateJun 17, 2019
AuthorsMirjam Hunziker / Jonas Barandun / Olga Buzovetsky / Caitlin Steckler / Henrik Molina / Sebastian Klinge /
PubMed AbstractEukaryotic ribosome biogenesis is initiated with the transcription of pre-ribosomal RNA at the 5' external transcribed spacer, which directs the early association of assembly factors but is absent ...Eukaryotic ribosome biogenesis is initiated with the transcription of pre-ribosomal RNA at the 5' external transcribed spacer, which directs the early association of assembly factors but is absent from the mature ribosome. The subsequent co-transcriptional association of ribosome assembly factors with pre-ribosomal RNA results in the formation of the small subunit processome. Here we show that stable rRNA domains of the small ribosomal subunit can independently recruit their own biogenesis factors in vivo. The final assembly and compaction of the small subunit processome requires the presence of the 5' external transcribed spacer RNA and all ribosomal RNA domains. Additionally, our cryo-electron microscopy structure of the earliest nucleolar pre-ribosomal assembly - the 5' external transcribed spacer ribonucleoprotein - provides a mechanism for how conformational changes in multi-protein complexes can be employed to regulate the accessibility of binding sites and therefore define the chronology of maturation events during early stages of ribosome assembly.
External linksElife / PubMed:31206356 / PubMed Central
MethodsEM (single particle)
Resolution4.3 Å
Structure data

EMDB-0441, PDB-6nd4:
Conformational switches control early maturation of the eukaryotic small ribosomal subunit
Method: EM (single particle) / Resolution: 4.3 Å

Source
  • saccharomyces cerevisiae by4741 (yeast)
  • Baker's yeast (baker's yeast)
KeywordsCryoelectron Microscopy / Eukaryotic Cells / Models, Molecular / Molecular Conformation / Protein Domains / RNA Precursors / RNA, Ribosomal, 18S / Ribosomal Proteins / Ribosome Subunits, Small / Ribosome Subunits, Small, Eukaryotic / Saccharomyces cerevisiae Proteins / RIBOSOME / Ribosome assembly

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